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Catherine Nury Sarah Bregant Bertrand Czarny Fannely Berthon Evelyne Cassar-Lajeunesse Vincent Dive 《The Journal of biological chemistry》2013,288(8):5636-5644
Matrix metalloproteases (MMPs) have attracted considerable attention as critical mediators of pathological tissue remodeling processes. However it remains an unresolved challenge to detect their active forms in biological samples. To prove the efficacy of a recently developed MMP activity-based probe, we examined the content in MMP active forms of bronchoalveolar lavage fluids (BALf) from male C57BL/6 mice exposed to ultrafine carbon black nanoparticles, a model of chronic obstructive pulmonary disease. This probe was shown to label proteins, mostly expressed in BALf of mice exposed to nanoparticles. Using competition assays with a selective MMP-12 inhibitor as well as MMP-12 knock-out mice, one of these proteins was identified as the active form of the catalytic domain of MMP-12. This new probe can detect the active form of MMP-12 down to a threshold of 1 fmol. Radioactive counting showed the concentration of the active form of MMP-12 to be around 1 fmol/μl in BALf from nanoparticle-treated mice. A less sensitive probe would therefore not have detected MMP-12. As the probe can detect other MMPs in the femtomolar range, it is a potentially powerful tool for monitoring the levels of MMP active forms in various diseases. 相似文献
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Maki Obara Hiroaki Satoh Yuko T Yamashita Hiroshi Kamma Morio Ohtsuka Shizuo Hasegawa Kazuo Orii 《Cancer immunology, immunotherapy : CII》1998,15(4):292-294
We report a case with metastatic small cell lung cancer which first manifested with biliary obstruction due to metastasis.
Prognosis of patients presenting with jaundice due to hepatic parenchyma involvement is thought to be poor. However, the patient
was successfully treated with percutaneous transhepatic biliary drainage and combination chemotherapy with reduced dosage.
We believe this to be the first such case report, despite the frequency of metastasis to the liver from small cell lung cancer. 相似文献